1) Use fresh feces as the test sample

　2) There is unknown infectivity when collecting samples, so they must be operated by trained or professional technicians

　2.Detection method

1) Preparation before testing: Test strips stored in the refrigerator should be placed at room temperature 15-30 minutes before testing to make the temperature consistent with room temperature. When the test sample is opened in a container that is cooler than room temperature, special attention should be paid to the humidity of the test paper due to the formation of water droplets.

2) Test strips stored at room temperature can be used directly.

3) Use a sampling stick to collect multiple samples from the surface and interior of the feces, or collect directly from the anus of the detection dog.

4) Place the sampling rod used to collect the sample into the collection tube, soak it in the buffer, and shake thoroughly.

5) Take out the test paper from the aluminum foil bag and place it on a dry and level surface.

6) Unscrew the buffer outlet and slowly and accurately drip 3 drops of detection solution into the sample hole.

7) Wait for the detection liquid to spread on the absorbent pad.

8) After about 5-10 minutes, the result can be judged when the control line (C) turns red. The longer the reaction time, the darker the color band between the control line and the detection line. Therefore, more accurate results can be obtained within a certain period of time after the control line completely turns red.

　3. Result judgment

Judge the result by whether color bands appear on the test line and the control line. Regardless of whether there is a canine parvovirus antigen control line (C) in the test sample, the color band will appear. If no color band appears, it is necessary to consider whether the operation was incorrect or whether there is a problem with the reagent and the test should be retested. Whether a color band appears on the test line determines whether the test sample contains canine parvovirus antigen.

1) Negative result: No color band appears on the test line (T), but a color band appears on the control line (C), which is judged as negative.

2) Color bands appear on both the test line (T) and the control line (C), which is considered positive.

3) There is no result and no color band appears on the test line (T) or the control line (C), or only the color band on the test line (T) appears, which is judged to be ineffective.

A. It is necessary to consider whether the operation is incorrect or whether there is a problem with the reagent, and the test should be re-tested.

B. When the titer of the canine distemper antigen in the test sample is particularly high, the test line is dark in color and the control line is light in color. In this case, the test sample needs to be diluted 10 times before testing, so that the test line (T) can be obtained The correct result of the color band appears with the control line (C).